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Ribas, Rodolfo Krüger da Câmara; Carboni, Diórgenes dos Santos; Cazarolli, Juciana Clarice; Flôres, Simone Hickmann; Ramirez-Castrillon, Maurício; Valente, Patricia. |
Lipases (E.C. 3.1.1.3) are serine-hydrolases, and act on long chain fatty acid ester bonds. They exhibit specific and enantioselective activities, which are desirable for many industrial applications. This study aimed at screening and optimizing the production of lipases by wild yeast strains from a variety of substrates, as well as characterizing the enzyme. An initial selection was made in oxygenated oil-supplemented minimum medium, and the enzymatic activity of the supernatant was tested over p- nitrophenyl palmitate. One-hundred and twenty-four yeast strains from different substrates were tested, and twenty-three showed significantly higher lipolytic activity (p <0.01). One yeast in particular, QU110, showed best lipase production and therefore... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: P-NPP; Tryptone; Yeast; Palmitate; Response Surface Model; Serine-hydrolase. |
Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/45481 |
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Li,H.B.; Yang,Y.R.Y.; Mo,Z.J.; Ding,Y.; Jiang,W.J.. |
The present study investigated the effect of silibinin, the principal potential anti-inflammatory flavonoid contained in silymarin, a mixture of flavonolignans extracted from Silybum marianum seeds, on palmitate-induced insulin resistance in C2C12 myotubes and its potential molecular mechanisms. Silibinin prevented the decrease of insulin-stimulated 2-NBDG (2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose) uptake and the downregulation of glutamate transporter type 4 (GLUT4) translocation in C2C12 myotubes induced by palmitate. Meanwhile, silibinin suppressed the palmitate-induced decrease of insulin-stimulated Akt Ser473 phosphorylation, which was reversed by wortmannin, a specific inhibitor of phosphatidylinositol-3-kinase (PI3K). We also... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Silibinin; Insulin resistance; Palmitate; C2C12. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500440 |
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